Blar i NIVA Open Access Archive på forfatter "Knudsen, Steen Wilhelm"

Viser treff 1-19 av 19

    • A baseline study of the occurrence of non-indigenous species in Danish harbours 

      Andersen, Jesper Harbo; Kallenbach, Emilie; Kjeldgaard, Mathias Brink; Knudsen, Steen Wilhelm; Eikrem, Wenche; Fagerli, Camilla With; Oug, Eivind; Dale, Trine; Thaulow, Jens; Gitmark, Janne Kim; Hobæk, Anders; Green, Norman Whitaker; Hesselsøe, Martin; Støttrup, Josianne; Kuhn, Jesper; Bekkevold, Dorte; Jacobsen, Lars Magnus Wulf; Møller, Peter Rask; Olesen, Christian Aakjær; Carl, Henrik; Stuer-Lauridsen, Frank (NIVA-rapport;7769-2022, Research report, 2022)
      We report the first ever nation-wide study of the occurrence of non-indigenous species in Danish harbours. The sampling was car-ried out using both conventional and biomolecular methods (eDNA). In total, 16 harbours were ...

    • Comparing DADA2 and OTU clustering approaches in studying the bacterial communities of atopic dermatitis 

      Barnes, Christopher J; Rasmussen, Linett; Asplund, Maria E; Knudsen, Steen Wilhelm; Clausen, Maja-Lisa; Agner, Tove; Hansen, Anders J (Peer reviewed; Journal article, 2020)
      Introduction. The pathogenesis of atopic dermatitis (AD) is not yet fully understood, but the bacterial composition of AD patients’ skin has been shown to have an increased abundance of Staphylococcus aureus. More recently, ...

    • Detektion af miljø-DNA med ‘quantitative Polymerase Chain Reaction’ (qPCR) sammenlignet med ‘droplet digital Polymerase Chain Reaction’ (ddPCR) 

      Knudsen, Steen Wilhelm (NIVA-rapport;7872-2023, Research report, 2023)
      Detektion af miljø-DNA (engelsk: ‘environmental DNA’, forkortet: ‘eDNA’) kan gøres med blandt andet qPCR og ddPCR, hvor ddPCR ved detektion af DNA fra virus eller bakterier i human patologiske studier er blevet demonstreret ...

    • Development of species-specific eDNA-based test systems for monitoring of freshwater crayfish 

      Knudsen, Steen Wilhelm; Agersnap, Sune; Møller, Peter Rask; Andersen, Jesper Harbo (NIVA-rapport;7447, Research report, 2019)
      This MONIS 5 report focuses on freshwater crayfish and was funded by the Danish Environmental Protection Agency. The aim of the present study was to develop species-specific systems for tracking environmental DNA (eDNA) ...

    • Development of species-specific eDNA-based test systems for monitoring of non-indigenous Decapoda in Danish marine waters 

      Knudsen, Steen Wilhelm; Møller, Peter Rask (NIVA-rapport;7544, Research report, 2020)
      We report the development of seven eDNA-based species-specific test systems for monitoring of marine Decapoda in Danish marine waters. The seven species are 1) Callinectes sapidus (blå svømmekrabbe), 2) Eriocheir sinensis ...

    • ECOMAR: A data-driven framework for ecosystem-based Maritime Spatial Planning in Danish marine waters. Results and conclusions from a development and demonstration project 

      Andersen, Jesper Harbo; Bendtsen, Jørgen; Hammer, Kathrine Jul; Harvey, E. Therese; Knudsen, Steen Wilhelm; Murray, Ciaran Joseph; Carstensen, Jacob; Petersen, Ib Krag; Svegaard, Signe; Tougaard, Jakob; Edelvang, Karen; Egekvist, Josefine; Olsen, Jeppe; Vinther, Morten; Al-Hamdani, Zyad; Jensen, Jørn Bo; Leth, Jørgen O; Kaae, Berit C; Olafsson, Anton Stahl; McClintock, William E.; Burt, Chad; Yocum, Dan (NIVA-rapport;7562, Research report, 2020)
      We report the developments and results of the ECOMAR project, which have taken place 2018-2020 and was funded by THE VELUX FOUNDATIONS. ECOMAR has established state-of-the-art data sets for the distribution of human ...

    • Ikke-hjemmehørende arter i marine områder 

      Andersen, Jesper Harbo; Knudsen, Steen Wilhelm; Murray, Ciaran Joseph; Carl, Henrik; Møller, Peter Rask; Hesselsøe, Martin (NIVA-rapport;7658, Research report, 2021)
      Denne rapport omhandler ikke-hjemmehørende arter i de danske farvande og består af to dele. Den første vedrører en opdate-ring af den nationale liste over ikke-hjemmhørende arter i de danske farvande – også kaldet NIS-listen. ...

    • Kortlægning af flodperlemusling i Varde Å-systemet ved brug af eDNA – en eftersøgning af nålen i høstakken 

      Rasmussen, Jes Jessen; Wyrwik, Christoffer; Sørensen, Mathias; Deacon, Michael; Sørensen, Flemming; Pedersen, Jan; Knudsen, Steen Wilhelm (NIVA-rapport;7818-2023, Research report, 2023)
      I dette studie blev der taget vandprøver i Varde Å, Linding Å, Ansager Å, Grindsted Å og Holme Å til kortlægning af forekomster af flodperlemusling ved brug af eDNA. Analyserne blev foretaget på en digital droplet PCR-platform, ...

    • Marine invasive alien species in Europe: 9 years after the IAS Regulation 

      Katsanevakis, Stelios; Olenin, Sergej; Puntila-Dodd, Riikka; Rilov, Gil; Stæhr, Peter A. U.; Teixeira, Heliana; Tsirintanis, Konstantinos; Birchenough, Silvana N. R.; Jakobsen, Hans H.; Knudsen, Steen Wilhelm; Lansén, Anders; Mazaris, Antonios D.; Piraino, Stefano; Tidbury, Hannah J. (Peer reviewed; Journal article, 2023)
      Biological invasions, resulting from human activities, exert substantial impacts on ecosystems worldwide. This review focuses on marine invasive alien species (IAS) in Europe, examining the current state, proposing strategies ...

    • Monitoring a Norwegian freshwater crayfish tragedy: eDNA snapshots of invasion, infection and extinction 

      Strand, David; Johnsen, Stein Ivar; Rusch, Johannes; Agersnap, Sune; Larsen, William Brenner; Knudsen, Steen Wilhelm; Møller, Peter Rask; Vrålstad, Trude (Journal article; Peer reviewed, 2019)
      1. The European noble crayfish Astacus astacus is threatened by crayfish plague caused by the oomycete Aphanomyces astaci, which is spread by the invasive North American crayfish (e.g. signal crayfish Pacifastacus leniusculus). ...

    • Monitoring of environmental DNA from nonindigenous species of algae, dinoflagellates and animals in the North East Atlantic 

      Knudsen, Steen Wilhelm; Hesselsøe, Martin; Thaulow, Jens; Agersnap, Sune; Hansen, Brian Klitgaard; Jacobsen, Magnus Wulff; Bekkevold, Dorte; Jensen, Søren K.S.; Møller, Peter Rask; Andersen, Jesper H. (Peer reviewed; Journal article, 2022)
      Monitoring the distribution of marine nonindigenous species is a challenging task. To support this monitoring, we developed and validated the specificity of 12 primer-probe assays for detection of environmental DNA (eDNA) ...

    • A National Scale “BioBlitz” Using Citizen Science and eDNA Metabarcoding for Monitoring Coastal Marine Fish 

      Agersnap, Sune; Sigsgaard, Eva Egelyng; Jensen, Mads Reinholdt; Ávila, Marcelo De Paula; Carl, Henrik; Møller, Peter Rask; Krøs, Simon Leed; Knudsen, Steen Wilhelm; Wisz, Mary S.; Thomsen, Philip Francis (Peer reviewed; Journal article, 2022)
      Marine biodiversity is threatened by human activities. To understand the changes happening in aquatic ecosystems and to inform management, detailed, synoptic monitoring of biodiversity across large spatial extents is needed. ...

    • Overvåking og kartlegging av fremmed ferskvannsfisk og vasspest ved bruk av miljø-DNA 

      Engesmo, Anette; Kistenich, Sonja; Knudsen, Steen Wilhelm; Christensen, Guttorm; Hesselsøe, Martin; Anglès d'Auriac, Marc (NIVA-rapport;7623, Research report, 2021)
      Tidligere publiserte molekylære deteksjonssystemer for fem invaderende fiskearter (pukkellaks, gjedde, ørekyt, sørv og suter) ble evaluert i henhold til Thalingers valideringsskala. Deretter ble det samlet miljø-DNA prøver ...

    • Overvåking og kartlegging av fremmed ferskvannsfisk ved bruk av miljø-DNA 

      Engesmo, Anette; Knudsen, Steen Wilhelm; Christensen, Guttorm; Hesselsøe, Martin; Anglès d'Auriac, Marc (NIVA-rapport;7435, Research report, 2019)
      Dette prosjektet er gjennomført på oppdrag fra Miljødirektoratet og målsetningen var å utvikle og teste ut et system for bruk av miljø-DNA som supplerende verktøy i overvåkning og kartlegging av fremmed ferskvannsfisk for ...

    • Overvåking og kartlegging av fremmed ferskvannsfisk ved bruk av miljø-DNA 2021 

      Engesmo, Anette; Egge, Elianne Dunthorn; Knudsen, Steen Wilhelm; Christensen, Guttorm; Hesselsøe, Martin; Anglès d'Auriac, Marc (NIVA-rapport;7732-2022, Research report, 2022)
      Det ble samlet miljø-DNA prøver fra Akersvannet og Virikbekken som ble analysert for gjedde og ørret. Resultatene viste et forutsigbart mønster i Virikbekken, med størst konsentrasjon av gjedde DNA øverst og størst ...

    • Remote, autonomous real‑time monitoring of environmental DNA from commercial fish 

      Hansen, Brian K; Jacobsen, Magnus W; Middelboe, Anne Lise; Preston, Christina M.; Marin III, Roman; Bekkevold, Dorte; Knudsen, Steen Wilhelm; Møller, Peter R.; Nielsen, Einar E (Peer reviewed; Journal article, 2020)
      Environmental DNA (eDNA) is increasingly used for monitoring marine organisms; however, offshore sampling and time lag from sampling to results remain problematic. In order to overcome these challenges a robotic sampler, ...

    • Short-term temporal variation of coastal marine eDNA 

      Jensen, Mads Reinholdt; Sigsgaard, Eva Egelyng; Ávila, Marcelo De Paula; Agersnap, Sune; Brenner-Larsen, William; Sengupta, Mita Eva; Xing, Yingchun; Krag, Marcus Anders; Knudsen, Steen Wilhelm; Carl, Henrik; Møller, Peter Daniel Rask; Thomsen, Philip Francis (Peer reviewed; Journal article, 2022)
      Temporal variation in eDNA signals is increasingly explored for understanding community ecology in aquatic habitats. Seasonal changes have been addressed using eDNA sampling, but very little is known regarding short-term ...

    • Supplementary material to ECOMAR: A data-driven framework for ecosystembased Maritime Spatial Planning in Danish marine waters 

      Andersen, Jesper Harbo; Hammer, Kathrine Jul; Harvey, E. Therese; Knudsen, Steen Wilhelm; Murray, Ciaran Joseph; Carstensen, Jacob; Petersen, Ib Krag; Sveegaard, Signe; Tougaard, Jakob; Edelvang, Karen; Olsen, Jeppe; Vinther, Morten; Al-Hamdani, Zyad; Jensen, Jørn Bo; Leth, Jørgen O; Kaae, Berit C; Olafsson, Anton S (NIVA-rapport;7525, Research report, 2020)
      This report contains, as said in the title, the supplementary material to the synthesis report from the ECOMAR project (2018- 2020). The supplementary material consists of three parts: 1) Annex A, being the data sets and ...

    • Tekniske anvisninger for eDNA-baseret overvågning af ikke-hjemmehørende marine arter 

      Knudsen, Steen Wilhelm; Andersen, Jesper Harbo; Bekkevold, Dorte; Hesselsøe, Martin; Jensen, Søren K S; Møller, Peter Rask (NIVA-rapport;7455, Research report, 2020)
      Disse tekniske anvisninger er en revideret udgave af de ansvisninger, der tidligere er udarbejdet for Miljøstyrelsen og publiceret vinteren 2017/2018. De reviderede tekniske anvisninger indeholder en detaljeret protokol ...