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dc.contributor.authorAnglès d'Auriac, Marc
dc.contributor.authorLe Gall, Line
dc.contributor.authorPeña, Viviana
dc.contributor.authorHall-Spencer, Jason M.
dc.contributor.authorSteneck, Robert S.
dc.contributor.authorFredriksen, Stein
dc.contributor.authorGitmark, Janne Kim
dc.contributor.authorChristie, Hartvig C
dc.contributor.authorHusa, Vivian
dc.contributor.authorGrefsrud, Ellen Sofie
dc.contributor.authorRinde, Eli
dc.date.accessioned2019-06-26T13:06:37Z
dc.date.available2019-06-26T13:06:37Z
dc.date.created2019-02-27T14:14:55Z
dc.date.issued2019
dc.identifier.citationScientific Reports. 2019, 9 (1), 578.nb_NO
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/11250/2602353
dc.description.abstractCoralline algae form extensive maerl and rhodolith habitats that support a rich biodiversity. Calcium carbonate harvesting as well as trawling activities threatens this ecosystem. Eleven species were recorded so far as maerl-forming in NE Atlantic, but identification based on morphological characters is unreliable. As for most red algae, we now use molecular characters to resolve identification of these taxa. However, obtaining DNA sequences requires time and resource demanding methods. The purpose of our study was to improve methods for achieving simple DNA extraction, amplification, sequencing and sequence analysis to allow robust identification of maerl species and other coralline algae. Our novel and easy DNA preparation method for coralline algae was of sufficient quality for qPCR amplification and sequencing of all 47 tested samples. The new psbA qPCR assay successfully amplified a 350 bp fragment identifying six species and uncovering two new Operational Taxonomic Units. Molecular results were corroborated with anatomical examination using i.e. scanning electron microscopy. Finally, the qPCR assay was coupled with High Resolution Melt analysis that successfully differentiated the closely related species Lithothamnion erinaceum and L. cf. glaciale. This DNA preparation and qPCR technique should vitalize coralline research by reducing time and cost associated with molecular systematics.nb_NO
dc.language.isoengnb_NO
dc.publisherNature Researchnb_NO
dc.relation.urihttps://doi.org/10.1038/s41598-018-36998-6
dc.rightsNavngivelse 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/deed.no*
dc.titleEfficient coralline algal psbA mini barcoding and High Resolution Melt (HRM) analysis using a simple custom DNA preparationnb_NO
dc.typeJournal articlenb_NO
dc.typePeer reviewednb_NO
dc.description.versionpublishedVersionnb_NO
dc.rights.holder© The Author(s) 2019nb_NO
dc.source.pagenumber9nb_NO
dc.source.volume9nb_NO
dc.source.journalScientific Reportsnb_NO
dc.source.issue1nb_NO
dc.identifier.doi10.1038/s41598-018-36998-6
dc.identifier.cristin1681066
cristin.unitcode7464,20,16,0
cristin.unitcode7464,20,11,0
cristin.unitnameSystemer og teknologi
cristin.unitnameMarin biologi
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1


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Navngivelse 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse 4.0 Internasjonal