Blar i NIVA Open Access Archive på forfatter "Larsen, Aud"
-
Application of flow cytometry in ballast water analysis - biological aspects
Hoell, Ingunn; Olsen, Ranveig Ottøy; Hess-Erga, Ole-Kristian; Thuestad, Gunnar; Larsen, Aud (Journal article; Peer reviewed, 2017)Ballast water may, when discharged, cause the spread of nonindigenous and potentially invasive species. International ballast water treatment regulations have accelerated the development of new methods to detect, enumerate ... -
Bioorthogonal Non-canonical Amino Acid Tagging Combined With Flow Cytometry for Determination of Activity in Aquatic Microorganisms
Lindivat, Mathilde; Larsen, Aud; Hess-Erga, Ole-Kristian; Bratbak, Gunnar; Hoell, Ingunn (Peer reviewed; Journal article, 2020)In this study, we have combined bioorthogonal non-canonical amino acid tagging (BONCAT) and flow cytometry (FCM) analysis, and we demonstrate the applicability of the method for marine prokaryotes. Enumeration of active ... -
Comparing flow cytometry and microscopy in the quantification of vital aquatic organisms in ballast water
Peperzak, Louis; Zetsche, Eva-Maria; Gollasch, Stephan; Artigas, Luis Felipe; Bonato, Simon; Créach, Véronique; Vré, Pieter de; Dubelaar, George B.J.; Henneghien, Joël; Hess-Erga, Ole-Kristian; Langelaar, Roland; Larsen, Aud; Maurer, Brian N; Mosselaar, Albert; Reavie, Euan D; Rijkeboer, Machteld; Tobiesen, August E.Dessen (Journal article; Peer reviewed, 2018)The ability to quantify vital aquatic organisms in the 2–50 µm size range was compared between five different flow cytometers and several different microscopes. Counts of calibration beads, algal monocultures of different ... -
Comparing flow cytometry and microscopy in the quantification of vital aquatic organisms in ballast water, Journal of Marine Engineering & Technology
Peperzak, Louis; Zetsche, Eva-Maria; Gollasch, Stephan; Artigas, Luis Felipe; Bonato, Simon; Créach, Véronique; Vré, Pieter de; Dubelaar, George B.J.; Henneghien, Joël; Hess-Erga, Ole-Kristian; Langelaar, Roland; Larsen, Aud; Maurer, Brian N; Mosselaar, Albert; Reavie, Euan D; Rijkeboer, Machteld; Tobiesen, August E.Dessen (Journal article; Peer reviewed, 2018)The ability to quantify vital aquatic organisms in the 2–50 µm size range was compared between five different flow cytometers and several different microscopes. Counts of calibration beads, algal monocultures of different ... -
Dual staining with CFDA-AM and SYTOX Blue in flow cytometry analysis of UV-irradiated Tetraselmis suecica to evaluate vitality
Olsen, Ranveig Ottøy; Hess-Erga, Ole-Kristian; Larsen, Aud; Hoffmann, Friederike; Thuestad, Gunnar; Hoell, Ingunn Alne (Journal article; Peer reviewed, 2016)After disinfection of ballast water, it is crucial to detect organisms and determine their vitality to assess the performance of the chosen treatment technique. Ultraviolet (UV) irradiation is a treatment technology commonly ... -
Flow Cytometric Analysis of Bacterial Protein Synthesis: Monitoring Vitality After Water Treatment
Lindivat, Mathilde; Bratbak, Gunnar; Larsen, Aud; Hess-Erga, Ole-Kristian; Hoell, Ingunn Alne (Peer reviewed; Journal article, 2021)Bacterial vitality after water disinfection treatment was investigated using bio-orthogonal non-canonical amino acid tagging (BONCAT) and flow cytometry (FCM). Protein synthesis activity and DNA integrity (BONCAT–SYBR ... -
Phytoplankton Blooms at Increasing Levels of Atmospheric Carbon Dioxide: Experimental Evidence for Negative Effects on Prymnesiophytes and Positive on Small Picoeukaryotes
Schulz, Kai G.; Bach, Lennart T.; Bellerby, Richard G. J; Bermúdez, Rafael; Büdenbender, Jan; Boxhammer, Tim; Czerny, Jan; Engel, Anja; Ludwig, Andrea; Meyerhöfer, Michael; Larsen, Aud; Paul, Allanah J.; Sswat, Michael; Riebesell, Ulf (Journal article; Peer reviewed, 2017)Anthropogenic emissions of carbon dioxide (CO2) and the ongoing accumulation in the surface ocean together with concomitantly decreasing pH and calcium carbonate saturation states have the potential to impact phytoplankton ... -
Seasonal diversity and dynamics of haptophytes in the Skagerrak, Norway, explored by high-throughput sequencing
Egge, Elianne Sirnæs; Johannessen, Torill Vik; Andersen, Tom; Eikrem, Wenche; Bittner, Lucie; Larsen, Aud; Sandaa, Ruth-Anne; Edvardsen, Bente (Journal article, 2015)Microalgae in the division Haptophyta play key roles in the marine ecosystem and in global biogeochemical processes. Despite their ecological importance, knowledge on seasonal dynamics, community composition and abundance ...