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dc.contributor.authorStrand, David
dc.contributor.authorJohnsen, Stein Ivar
dc.contributor.authorRusch, Johannes
dc.contributor.authorAgersnap, Sune
dc.contributor.authorLarsen, William Brenner
dc.contributor.authorKnudsen, Steen Wilhelm
dc.contributor.authorMøller, Peter Rask
dc.contributor.authorVrålstad, Trude
dc.date.accessioned2019-10-08T07:16:40Z
dc.date.available2019-10-08T07:16:40Z
dc.date.created2019-05-29T18:00:12Z
dc.date.issued2019
dc.identifier.citationJournal of Applied Ecology. 2019, 56 (7), 1661-1673.nb_NO
dc.identifier.issn0021-8901
dc.identifier.urihttp://hdl.handle.net/11250/2620733
dc.description.abstract1. The European noble crayfish Astacus astacus is threatened by crayfish plague caused by the oomycete Aphanomyces astaci, which is spread by the invasive North American crayfish (e.g. signal crayfish Pacifastacus leniusculus). Surveillance of crayfish plague status in Norway has traditionally relied on the monitoring survival of cage‐held noble crayfish, a method of ethical concern. Additionally, trapping is used in crayfish population surveillance. Here, we test whether environmental DNA (eDNA) monitoring could provide a suitable alternative to the cage method, and a supplement to trapping. 2. We took advantage of an emerging crayfish plague outbreak in a Norwegian watercourse following illegal introduction of disease‐carrying signal crayfish, and initiated simultaneous eDNA monitoring and cage‐based surveillance, supplemented with trapping. A total of 304 water samples were filtered from several sampling stations over a 4‐year period. eDNA data (species‐specific quantitative real‐time PCR [qPCR]) for the presence of A. astaci, noble and signal crayfish within the water samples were compared to cage mortality and trapping. 3. This is the first study comparing eDNA monitoring and cage surveillance during a natural crayfish plague outbreak. We show that eDNA monitoring corresponds well with the biological status measured in terms of crayfish mortality and trapping results. eDNA analysis also reveals the presence of A. astaci in the water up to 2.5 weeks in advance of the cage method. Estimates of A. astaci and noble crayfish eDNA concentrations increased markedly during mortality and vanished quickly thereafter. eDNA provides a snapshot of the presence, absence or disappearance of crayfish regardless of season, and constitutes a valuable supplement to the trapping method that relies on season and legislation. 4. Synthesis and applications. Simultaneous eDNA monitoring of Aphanomyces astaci (crayfish plague) and relevant native and invasive freshwater crayfish species is well‐suited for early warning of invasion or infection, risk assessments, habitat evaluation and surveillance regarding pathogen and invasive/native crayfish status. This non‐invasive, animal welfare friendly method excludes the need for cage‐held susceptible crayfish in disease monitoring. Furthermore, eDNA monitoring is less likely to spread A. astaci than traditional methods. This study resulted in the implementation of eDNA monitoring for Norwegian crayfish plague and crayfish surveillance programmes, and we believe other countries could improve management strategies for freshwater crayfish using a similar approach.nb_NO
dc.language.isoengnb_NO
dc.relation.urihttps://datadryad.org/resource/doi:10.5061/dryad.vf86jb2
dc.rightsNavngivelse 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/deed.no*
dc.subjectKrepsdyrnb_NO
dc.subjectCrustaceanb_NO
dc.subjectAkvatisk faunanb_NO
dc.subjectAquatic faunanb_NO
dc.subjectOomyceter / Eggsporesoppernb_NO
dc.subjectOomycetesnb_NO
dc.subjectKrepsepestnb_NO
dc.subjectCrayfish plaguenb_NO
dc.subjectMiljø DNAnb_NO
dc.subjectEnvironmental DNAnb_NO
dc.subjectEDNA baserte metodernb_NO
dc.subjectEDNA based methodsnb_NO
dc.subjectAkvatisk miljønb_NO
dc.subjectAquatic environmentnb_NO
dc.subjectTifotkrepsnb_NO
dc.subjectDecapod crustaceansnb_NO
dc.titleMonitoring a Norwegian freshwater crayfish tragedy: eDNA snapshots of invasion, infection and extinctionnb_NO
dc.typeJournal articlenb_NO
dc.typePeer reviewednb_NO
dc.description.versionpublishedVersionnb_NO
dc.rights.holder© 2019 The Authorsnb_NO
dc.subject.nsiVDP::Basale medisinske, odontologiske og veterinærmedisinske fag: 710nb_NO
dc.subject.nsiVDP::Basic medical, dental and veterinary sciences: 710nb_NO
dc.source.pagenumber1661-1673nb_NO
dc.source.volume56nb_NO
dc.source.journalJournal of Applied Ecologynb_NO
dc.source.issue7nb_NO
dc.identifier.doi10.1111/1365-2664.13404
dc.identifier.cristin1701469
dc.relation.projectNorges forskningsråd: 243907nb_NO
cristin.unitcode7464,30,19,0
cristin.unitnameFerskvannsøkologi
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode2


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