Show simple item record

dc.contributor.authorOlsen, Ranveig Ottøy
dc.contributor.authorHess-Erga, Ole-Kristian
dc.contributor.authorLarsen, Aud
dc.contributor.authorHoffmann, Friederike
dc.contributor.authorThuestad, Gunnar
dc.contributor.authorHoell, Ingunn Alne
dc.date.accessioned2018-06-13T10:26:21Z
dc.date.available2018-06-13T10:26:21Z
dc.date.created2016-10-19T13:33:37Z
dc.date.issued2016
dc.identifier.citationAquatic Biology. 2016, 25, 39-52.nb_NO
dc.identifier.issn1864-7790
dc.identifier.urihttp://hdl.handle.net/11250/2501404
dc.description.abstractAfter disinfection of ballast water, it is crucial to detect organisms and determine their vitality to assess the performance of the chosen treatment technique. Ultraviolet (UV) irradiation is a treatment technology commonly used for water disinfection. In this study, the phytoplankter Tetraselmis suecica was UV irradiated and subsequently stained with both 5-carboxyfluorescein diacetate acetoxymethyl ester (CFDA-AM) and SYTOX Blue, staining metabolically active and membrane-permeable cells, respectively. This dual staining protocol can be used to assess samples during type approval of UV-based treatment systems. Non-irradiated and UV-irradiated samples were incubated in darkness, to simulate a ballast water transport, after which the vitality and viability T. suecica were monitored regularly over a period of 15 d. Flow cytometry (FCM) analysis separated the cells into 4 FCM populations (=single cells grouped together based on their fluorescence signals) according to differences in esterase activity and membrane integrity. UV-irradiated samples followed a different staining pattern compared to non-irradiated samples, where 1 specific FCM population of cells expressed esterase activity, but at the same time gave signals for disrupted membranes. This is useful as a sign of future death and is interpreted as an ‘early warning’ FCM population. FCM results were also compared to corresponding plate count results, differentiating vital, viable cells from vital, non-viable cells. We argue that dual staining with SYTOX Blue and CFDA-AM facilitates and improves FCM analysis when evaluating the performance of UV-based water treatment systems.nb_NO
dc.language.isoengnb_NO
dc.publisherInter Researchnb_NO
dc.rightsNavngivelse 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/deed.no*
dc.titleDual staining with CFDA-AM and SYTOX Blue in flow cytometry analysis of UV-irradiated Tetraselmis suecica to evaluate vitalitynb_NO
dc.typeJournal articlenb_NO
dc.typePeer reviewednb_NO
dc.description.versionpublishedVersionnb_NO
dc.rights.holder© The authors 2016nb_NO
dc.source.pagenumber39-52nb_NO
dc.source.volume25nb_NO
dc.source.journalAquatic Biologynb_NO
dc.identifier.doi10.3354/ab00662
dc.identifier.cristin1392933
dc.relation.projectNorges forskningsråd: 208653nb_NO
cristin.unitcode7464,20,15,0
cristin.unitnameAkvakultur
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

Navngivelse 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse 4.0 Internasjonal