dc.contributor.author | Sandvik, Morten | |
dc.contributor.author | Waaler, Thor Arnulf | |
dc.contributor.author | Rundberget, Thomas | |
dc.contributor.author | Adolfsen, Pål | |
dc.contributor.author | Bardal, Helge | |
dc.contributor.author | Sandodden, Roar | |
dc.date.accessioned | 2019-06-27T06:55:38Z | |
dc.date.available | 2019-06-27T06:55:38Z | |
dc.date.created | 2017-12-12T12:15:49Z | |
dc.date.issued | 2018 | |
dc.identifier.citation | Management of Biological Invasions. 2018, 9 (1), 59-65. | nb_NO |
dc.identifier.issn | 1989-8649 | |
dc.identifier.uri | http://hdl.handle.net/11250/2602428 | |
dc.description.abstract | A fast, accurate and simple method using liquid chromatography (LC) with UV detection was used for the on-site determination of the piscicide rotenonein water during fish control treatments. Sample volumes of 10 to 40 µL were loaded onto a Waters XBridge™ C18 2.5 µm 3.0 x100 mm analytical column usinga mobile phase of water–acetonitrile (45:55) at a flow-rate of 0.5 mL/min. The method was evaluated using river and estuarine water spiked with rotenone(0.1–330 µg/L) and various preservation methods. The within-assay precision measured as relative standard deviation (RSD, n = 12) was 5.5 to 6.5% andthe between assay precision (RSD, n = 4) was 6.5 to 7.5%. The limit of quantification was 1 µg/L, below normal piscicidal treatment rates (5 to 200 µg/L)and regulatory limits (< 2 µg/L) generally considered safe. The analysis time was 6 min/sample allowing for real-time adjustment of rotenone dosages duringfish control treatments. The relatively small size (75×60×50 cm) of the LC system made it ideal for transportation and installation in remote treatment areas;it can be operated out of a small trailer in the field with electricity. Our studies indicate that the preservation of water samples with equal quantitiesof acetonitrile stabilizes rotenone indefinitely (> 170 days) if kept cool (4 °C) and in the dark. Although increased salinity decreased the recoveryof rotenone, sample filtration with Spin-X filter membranes negated the effect. | nb_NO |
dc.language.iso | eng | nb_NO |
dc.publisher | Regional Euro-Asian Biological Invasions Centre | nb_NO |
dc.rights | Navngivelse 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/deed.no | * |
dc.title | Fast and accurate on-site determination of rotenone in water during fish control treatments using liquid chromatography | nb_NO |
dc.type | Journal article | nb_NO |
dc.type | Peer reviewed | nb_NO |
dc.description.version | publishedVersion | nb_NO |
dc.rights.holder | © 2018 The Author(s). | nb_NO |
dc.source.pagenumber | 59-65 | nb_NO |
dc.source.volume | 9 | nb_NO |
dc.source.journal | Management of Biological Invasions | nb_NO |
dc.source.issue | 1 | nb_NO |
dc.identifier.doi | 10.3391/mbi.2018.9.1.06 | |
dc.identifier.cristin | 1526229 | |
cristin.unitcode | 7464,30,21,0 | |
cristin.unitname | Miljøkjemi | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |