No evidence of a protective or cumulative negative effect of UV-B on growth inhibition induced by gamma radiation in Scots pine (Pinus sylvestris) seedlings
Blagojevic, Dajana; Lee, YeonKyeong; Xie, Li; Brede, Dag A; Nybakken, Line; Lind, Ole Christian; Tollefsen, Knut Erik; Salbu, Brit; Solhaug, Knut Asbjørn; Olsen, Jorunn E
Peer reviewed, Journal article
Accepted version
Date
2019Metadata
Show full item recordCollections
- Publikasjoner fra Cristin - NIVA [2255]
- Scientific publications [1254]
Original version
Photochemical and Photobiological Sciences. 2019, 18 (8), 1945-1962. 10.1039/c8pp00491aAbstract
Exposure to ambient UV-B radiation may prime protective responses towards various stressors in plants, though information about interactive effects of UV-B and gamma radiation is scarce. Here, we aimed to test whether UV-B exposure could prime acclimatisation mechanisms contributing to tolerance to low-moderate gamma radiation levels in Scots pine seedlings, and concurrently whether simultaneous UV-B and gamma exposure may have an additive adverse effect on seedlings that had previously not encountered either of these stressors. Responses to simultaneous UV-B (0.35 W m−2) and gamma radiation (10.2–125 mGy h−1) for 6 days with or without UV-B pre-exposure (0.35 W m−2, 4 days) were studied across various levels of organisation, as compared to effects of either radiation type. In contrast to UV-B, and regardless of UV-B presence, gamma radiation at ≥42.9 mGy h−1 caused increased formation of reactive oxygen species and reduced shoot length, and reduced root length at 125 mGy h−1. In all experiments there was a gamma dose rate-dependent increase in DNA damage at ≥10.8 mGy h−1, generally with additional UV-B-induced damage. Gamma-induced growth inhibition and gamma- and UV-B-induced DNA damage were still visible 44 days post-irradiation, even at 20.7 mGy h−1, probably due to genomic instability, but this was reversed after 8 months. In conclusion, there was no evidence of a protective effect of UV-B on gamma-induced growth inhibition and DNA damage in Scots pine, and no additive adverse effect of gamma and UV-B radiation on growth in spite of the additional UV-B-induced DNA damage.
Description
Embargo until 08 July 2020