Multi-laboratory validation of a new marine biodegradation screening test for chemical persistence assessment
Ott, Amelie; Martin, Timothy J.; Acharya, Kishor; Lyon, Delina Y.; Robinson, Nik; Rowles, Bob; Snape, Jason R.; Still, Ian; Whale, Graham F.; Albright, Vurtice C.; Bäverbäck, Petra; Best, Nicola; Commander, Ruth; Eickhoff, Curtis; Finn, Sarah; Hidding, Björn; Maischak, Heiko; Sowders, Katherine A.; Taruki, Masanori; Walton, Helen E.; Wennberg, Aina Charlotte; Davenport, Russell J.
Peer reviewed, Journal article
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Original versionEnvironmental Science and Technology. 2020, 54 (7), 4210-4220. 10.1021/acs.est.9b07710
Current biodegradation screening tests are not specifically designed for persistence assessment of chemicals, often show high inter- and intra-test variability, and often give false negative biodegradation results. Based on previous studies and recommendations, an international ring test involving 13 laboratories validated a new test method for marine biodegradation with a focus on improving the reliability of screening to determine the environmental degradation potential of chemicals. The new method incorporated increased bacterial cell concentrations to better represent the microbial diversity; a chemical is likely to be exposed in the sampled environments and ran beyond 60 days, which is the half-life threshold for chemical persistence in the marine environment. The new test provided a more reliable and less variable characterization of the biodegradation behavior of five reference chemicals (sodium benzoate, triethanolamine, 4-nitrophenol, anionic polyacrylamide, and pentachlorophenol), with respect to REACH and OSPAR persistence thresholds, than the current OECD 306 test. The proposed new method provides a cost-effective screening test for non-persistence that could streamline chemical regulation and reduce the cost and animal welfare implications of further higher tier testing.
Embargo until 12 March 2021